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    THẠC SĨ Purification and Antioxidant Activities of Soybean Isoflavones

    Ác Niệm Ác Niệm Đang Ngoại tuyến (3588 tài liệu)
    .:: Gold Member ::.
  1. Gửi tài liệu
  2. Bình luận
  3. Chia sẻ
  4. Thông tin
  5. Công cụ
  6. Purification and Antioxidant Activities of Soybean Isoflavones

    Table of Contents
    List of Tables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
    List of Figures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vi
    Chapter 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
    Chapter 2 Overview of the Mechanisms for Soybean Isoflavones’ Health
    Improvement Actions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
    5
    2.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
    2.2 Anticancer Actions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
    2.2.1 Cancer Preventive Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
    2.2.2 Cancer Inhibitory Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
    2.3 Cardiovascular System Protective Actions . . . . . . . . . . . . . . . . . . . . . . . 16
    2.4 Anti-osteoporosis Actions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
    2.5 Other Applications of Soybean Isoflavones . . . . . . . . . . . . . . . . . . . . . . . 20
    2.6 Future Implications of Soybean Isoflavones . . . . . . . . . . . . . . . . . . . . . . 21
    Chapter 3 Materials and Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
    3.1 Extraction and Purification of Soybean Isoflavones . . . . . . . . . . . . . . . 23
    3.1.1 Chemicals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
    3.1.2 Extraction of Soybean Isoflavones . . . . . . . . . . . . . . . . . . . . . . . . 23
    3.1.3 Purification and Analysis of Daidzin, Glycitin, and Genistin . . . 23
    3.2 Antioxidant Activities of Soybean Isoflavones in Preventing
    Cholesterol Oxidation Accelerated by 2,2’-Azobis(2-
    methylpropionamidine) Dihydrochloride. . . . . . . . . . . . . . . . . . . . . . . .
    24
    3.2.1 Chemicals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
    3.2.2 Cholesterol Oxidation System . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
    3.2.3 Cholesterol Oxidation System Treatment with Soybean
    Isoflavones . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
    25
    3.2.4 Statistical Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
    3.3 Antioxidant Activities of Soybean Isoflavones on tert-Butyl
    Hydroperoxide-Induced Oxidative Stress in SVEC4-10 Cells . . . . . . .
    26
    3.3.1 Chemicals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
    3.3.2 Cell Culture and Cell Growth Curve . . . . . . . . . . . . . . . . . . . . . . . . 26
    3.3.3 Cytotoxicity Study of Genistein and Daidzein . . . . . . . . . . . . . . . . 27
    3.3.4 Cytotoxicity Study of tBOOH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
    3.3.5 Cell Treatment with Soybean Isoflavones. . . . . . . . . . . . . . . . . . . . 28
    3.3.6 Statistical Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
    Acknowledgments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ii
    Abstract . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii
    iv
    Chapter 4 Results and Discussion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
    4.1 Extraction and Purification of Soybean Isoflavones . . . . . . . . . . . . . . . . 30
    4.1.1 Purification and Analysis of Daidzin, Glycitin, and Genistin . . . . 30
    4.2 Antioxidant Activities of Soybean Isoflavones in Preventing
    Cholesterol Oxidation Accelerated by 2,2’-Azobis(2-
    methylpropionamidine) Dihydrochloride . . . . . . . . . . . . . . . . . . . . . . .
    30
    4.2.1 Cholesterol Oxidation Products with AAPH Acceleration . . . . . . 30
    4.2.2 Antioxidant Activities of Genistein, Daidzein, and Glycosides
    Mixture from Defatted Soybean Flour . . . . . . . . . . . . . . . . . . . .
    34
    4.3 Antioxidant Activities of Soybean Isoflavones on tert-Butyl
    Hydroperoxide-Induced Oxidative Stress in SVEC4-10 Cells . . . . . . .
    42
    4.3.1 Growth Curve of SVEC4-10 Cells . . . . . . . . . . . . . . . . . . . . . . . . 42
    4.3.2 Cytotoxicity of Genistein and Daidzein . . . . . . . . . . . . . . . . . . . . 43
    4.3.3 Cytotoxicity of tBOOH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
    4.3.4 Antioxidant Activities of Genistein and Daidzein . . . . . . . . . . . . 46
    Chapter 5 Conclusion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
    References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
    Vita . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

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